Betacellulin, Recombinant, Human, aa32-111, His-Tag

Article Name:	Betacellulin, Recombinant, Human, aa32-111, His-Tag
Biozol Catalog Number:	USB-497018
Supplier Catalog Number:	497018
Alternative Catalog Number:	USB-497018-10,USB-497018-20,USB-497018-50,USB-497018-100,USB-497018-250
Manufacturer:	US Biological
Category:	Molekularbiologie

BTC, also known as Betacellulin, is a member of epidermal growth factor receptor family and functions as a ligand for the epidermal growth factor receptor (EGFR). As the role a EGFR, betacellulin is manifested by different form of muscles and tissues, it also has a great effect of nitrogen that is used for retinal pigment epithelial cells and vascular smooth muscle cells. While many studies attest a role for betacellulin in the differentiation of pancreatic beta-cells, the last decade witnessed the association of betacellulin with many additional biological processes, ranging from reproduction to the control of neural stem cells. As a typical EGFR ligand, betacellulin is expressed by a variety of cell types and tissues, and the soluble growth factor is proteolytically cleaved from a larger membrane-anchored precursor. It stimulates the proliferation of retinal pigment epithelial and vascular smooth muscle cells but did not stimulate the growth of several other cell types, such as endothelial cells and fetal lung fibroblasts. Recombinant protein corresponding to aa32-111 from human Betacellulin, fused to His-Tag at C-terminal, expressed in HEK293 cells. Molecular Weight: ~9.8kD Biological Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range 0.5ng/ml. Amino Acid Sequence: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:	9.886
NCBI:	001720
UniProt:	P35070
Purity:	95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
Form:	Supplied as a liquid in PBS, pH 7.4, 10% glycerol