CD40 Ligand, Recombinant, Human, aa113-261, His-Tag

Product Overview

• Article Name: CD40 Ligand, Recombinant, Human, aa113-261, His-Tag
• Biozol Catalog Number: USB-497026
• Supplier Catalog Number: 497026
• Alternative Catalog Number: USB-497026-10,USB-497026-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

CD40 ligand, also known as CD40LG, is a member of the TNF superfamily and is expressed on activated T cells. CD40LG is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. Recombinant protein corresponding to aa113-261 from human CD40 Ligand/TNFSF5, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~18.3kD Biological Activity: Measured by its binding ability in a functional ELISA with Human CD40/TNFRSF5. Amino Acid Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 18.3169
• NCBI: 000065
• UniProt: P29965
• Purity: 95% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.2M sodium chloride, 40% glycerol.