DC-SIGN/CD209, Recombinant, Human, aa60-404, hIgG-His-Tag

Article Name:	DC-SIGN/CD209, Recombinant, Human, aa60-404, hIgG-His-Tag
Biozol Catalog Number:	USB-497033
Supplier Catalog Number:	497033
Alternative Catalog Number:	USB-497033-10,USB-497033-20,USB-497033-50,USB-497033-100,USB-497033-250
Manufacturer:	US Biological
Category:	Molekularbiologie

DC-SIGN/CD209, also known as dendritic cell-specific ICAM-3 grabbing non-integrin, is a member of the C-type lectin family. It is pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. DC-SIGN on macrophages recognises and binds with high affinity to high-mannose type N-glycans, a class of pathogen associated molecular patterns PAMPs commonly found on viruses, bacteria and fungi. This binding interaction activates phagocytosis. It has a high affinity for the ICAM3 molecule and binds various microorganisms by recognizing high-mannose-containing glycoproteins on their envelopes and especially functions as receptor for several viruses such as HIV and Hepatitis C. Besides functioning as an adhesion molecule, recent study has also shown that DC-SIGN can initiate innate immunity by modulating toll-like receptors, though the detailed mechanism is not yet known. It together with other C-type lectins is involved in recognition of tumors by dendritic cells. It is also a potential engineering target for dendritic cell based cancer vaccine. Recombinant protein corresponding to aa60-404 from human DC-SIGN/CD209, fused to hIgG-His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~66.5kD Amino Acid Sequence: VSKVPSS ISQEQSRQDA IYQNLTQLKA AVGELSEKSK LQEIYQELTQ LKAAVGELPE KSKLQEIYQE LTRLKAAVGE LPEKSKLQEI YQELTWLKAA VGELPEKSKM QEIYQELTRL KAAVGELPEK SKQQEIYQEL TRLKAAVGEL PEKSKQQEIY QELTRLKAAV GELPEKSKQQ EIYQELTQLK AAVERLCHPC PWEWTFFQGN CYFMSNSQRN WHDSITACKE VGAQLVVIKS AEEQNFLQLQ SSRSNRFTWM GLSDLNQEGT WQWVDGSPLL PSFKQYWNRG EPNNVGEEDC AEFSGNGWND DKCNLAKFWI CKKSAASCSR DEEQFLSPAP ATPNPPPA Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:	66.5587
NCBI:	066978
UniProt:	Q9NNX6
Purity:	90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
Form:	Supplied as a liquid in PBS, pH 7.4, 10% glycerol