IL-1 beta/IL-1F2, Recombinant, Canine, aa114-265
Catalog Number:
USB-497042
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| Article Name: | IL-1 beta/IL-1F2, Recombinant, Canine, aa114-265 |
| Biozol Catalog Number: | USB-497042 |
| Supplier Catalog Number: | 497042 |
| Alternative Catalog Number: | USB-497042-10,USB-497042-20 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa114-265 from canine IL-1 beta/IL-1F2, expressed in E. coli. Molecular Weight: ~17.5kD (153aa) confirmed by MALDI-TOF Biological Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range 5pg/ml. Amino Acid Sequence: MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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