PSGL-1, Recombinant, Human, aa42-295, hIgG-His-Tag

Product Overview

• Article Name: PSGL-1, Recombinant, Human, aa42-295, hIgG-His-Tag
• Biozol Catalog Number: USB-497069
• Supplier Catalog Number: 497069
• Alternative Catalog Number: USB-497069-10,USB-497069-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

PSGL-1, also known CD162 or SELPLG, is a mucin-type glycoprotein that functions as a high affinity counterreceptor for the cell adhesion molecules P-, E and L- selectin expressed on myeloid cells and stimulated T lymphocytes. This protein plays a key role in leukocyte adhesion. As such, it plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. PSGL-1 binds chmokines such as CCL19, CCL21, and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Recombinant protein corresponding to aa42-395 from human PSGL-1/CD162, fused to hIgG-His-Tag at C-terminal, expressed in HEK293 cells. Molecular Weight: ~53.4kD Amino Acid Sequence: QATEYEY LDYDFLPETE PPEMLRNSTD TTPLTGPGTP ESTTVEPAAR RSTGLDAGGA VTELTTELAN MGNLSTDSAA MEIQTTQPAA TEAQTTPLAA TEAQTTRLTA TEAQTTPLAA TEAQTTPPAA TEAQTTQPTG LEAQTTAPAA MEAQTTAPAA MEAQTTPPAA MEAQTTQTTA MEAQTTAPEA TEAQTTQPTA TEAQTTPLAA MEALSTEPSA TEALSMEPTT KRGLFIPFSV SSVTHKGIPM AASNLSV Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 53.4496
• NCBI: 50061
• UniProt: Q14242
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL)
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol