| The Integral membrane protein 2B (ITM2B) BioAssay(TM) ELISA Kit is a sandwich immunoassay for the in vitro quantitative measurement of human ITM2B concentrations in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Detection Range: 0.78-50ng/ml Sensitivity: <0.4ng/ml Precision: Intra-Assay CV: <6% Inter-Assay CV: <11% Assay Principle: An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient ITM2B will produce a blue colored product, which then changes to yellow after adding the acidic stop solution. The intensity of the yellow color is proportional to the ITM2B amount bound on the plate. The Optical Density (OD) is measured spectrophotometrically at 450nm in a microplate reader, from which the concentration of ITM2B can be calculated. Kit Components: 497277A: Microtiter Plate, 1x96 wells 497277B: Standard, 2x1 vial 497277C: Sample/Standard Diluent, 1x20ml 497277D: Detection Reagent A, 100X 1x120ul 497277E: Detection Reagent B, 100X, 1x120ul 497277F: Diluent A, 1x10ml 497277G: Diluent B, 1x10ml *497277H: TMB Substrate, 1x10ml *497277K: Stop Solution, 1x10ml *497277L: Wash Buffer, 25X, 1x30ml Precaution: The Stop Solution (497277K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *497277H, *497277K, *497277L at 4C. Store all the other components at -20C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. |
