Mouse Interleukin-22 (IL-22), also known as T cell-derived inducible factor (ILTIF), is a cytokine that is structurally related to IL-10. Studies have shown that IL-22 induces acute-phase reactants in vitro and in vivo, suggesting that the cytokine contributes to the regulation of inflammatory responses. Source: Recombinant protein corresponding to two 146aa chains from mouse IL-22, expressed in E. coli. Applications: Positive Control, Immunogen, SDS-PAGE, WB. If bio-activity of the protein is needed, please see active protein catalog no: 21993. Biological Activity: Fully biologically active when compared to a standard. The ED50, as determined by its ability to induce IL-10 secretion in Colo205 cells, is <0.5ng/ml, corresponding to a specific activity of 2x10e6IU/mg Subcellular Location: Secreted Predicted Molecular Mass: 20.5kD Residues & Tags: Leu34~Val179 (Accession Q9JJY9) with N-terminal His Tag Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LPVNTRC KLEVSNFQQP YIVNRTFMLA KEASLADNNT DVRLIGEKLF RGVSAKDQCY LMKQVLNFTL EDVLLPQSDR FQPYMQEVVP FLTKLSNQLS SCHISGDDQN IQKNVRRLKE TVKKLGESGE IKAIGELDLL FMSLRNACV Endotoxin: <0.1ng/ug Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage conditions. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
