Elongin B, Recombinant, Mouse, aa1-118, His-Tag (Elob)

Product Overview

• Article Name: Elongin B, Recombinant, Mouse, aa1-118, His-Tag (Elob)
• Biozol Catalog Number: USB-506168
• Supplier Catalog Number: 506168
• Alternative Catalog Number: USB-506168-50,USB-506168-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Elob, also known as elongin B, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimeric consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant protein corresponding to aa1-118 of mouse Elob, fused to His-tag at N-terminus, expressed in E. coli. Molecular Weight: ~15.6kD (141aa), confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDVFLMI RRHKTTIFTD AKESSTVFEL KRIVEGILKR PPEEQRLYKD DQLLDDGKTL GECGFTSQTA RPQAPATVGL AFRADDTFEA LRIEPFSSPP ELPDVMKPQD SGGSANEQAV Q Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 15.6141
• NCBI: 080581
• UniProt: P62869
• Purity: 95% (SDS-PAGE)
• Form: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 10% glycerol.