TNNI3, also known as Troponin I3, is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TNNI3 is responsible for inhibition of actomyosin ATPase activity. Therefore, TNNI3 is a key regulatory protein in cardiac muscle contraction and relaxation cycle. Recently, specific missense mutations of the TNNI3 gene have been shown to cause familial hypertrophic cardiomyopathy. Recombinant human TNNI3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography. Recombinant protein corresponding to aa1-210 of human TNNI3, fused to His-tag at N-terminus, expressed in E. coli. Molecular Weight: ~26.4kD (233aa) confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADGSSD AAREPRPAPA PIRRRSSNYR AYATEPHAKK KSKISASRKL QLKTLLLQIA KQELEREAEE RRGEKGRALS TRCQPLELAG LGFAELQDLC RQLHARVDKV DEERYDIEAK VTKNITEIAD LTQKIFDLRG KFKRPTLRRV RISADAMMQA LLGARAKESL DLRAHLKQVK KEDTEKENRE VGDWRKNIDA LSGMEGRKKK FES Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
