MAGE Family Member C1 (MAGEC1) BioAssay(TM) ELISA Kit (Human)
Biozol Catalog Number:
USB-516564
Supplier Catalog Number:
516564
Alternative Catalog Number:
USB-516564-96
Manufacturer:
US Biological
Category:
Kits/Assays
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids Sensitivity: <0.065ng/ml Range: 0.15-10ng/ml Precision: Intra-Assay: <5.5% (n = 20) Inter-Assay: <10.2% (n = 20) Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% Test Principle: The MAGEC1 ELISA Kit (Human) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MAGEC1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human MAGEC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450nm is quantitatively proportional to the amount of sample Human MAGEC1 captured in well. Kit Components: MAGEC1 Microplate, 96 Wells MAGEC1 Lyophilized Standard, 2x vial 100X Biotinylated MAGEC1 Detector Antibody, 1x120ul 100X Avidin-HRP Conjugate, 1x120ul Sample Diluent, 1x20ml Detector Antibody Diluent, 1x12ml Conjugate Diluent, 1x12ml 25X Wash Buffer , 1x30ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
