sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
Recombinant monoclonal antibody to CD40. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5D12. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis. Applications: Suitable for use in Blocking, Immunoprecipitation, ELISA, Flow Cytometry, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
