Activated Protein C (APC) High Sensitivity BioAssay(TM) ELISA Kit (Human)
Biozol Catalog Number:
USB-516952
Supplier Catalog Number:
516952
Alternative Catalog Number:
USB-516952-96
Manufacturer:
US Biological
Category:
Kits/Assays
Sample Type: Plasma Intended Use: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of APC in human plasma. Sensitivity: 5.6pg/ml Range: 15.6-1000pg/ml Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Activated Protein C (APC). No significant cross-reactivity or interference between High Sensitive Activated Protein C (APC) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to APC. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to APC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of APC in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1*120ul Detection Reagent B, 1*120ul TMB Substrate, 1*9ml Wash Buffer (30X), 1*20ml Standard Diluent, 1*20ml Assay Diluent A, 1*12ml Assay Diluent B, 1*12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
