Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Intended Use: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of obestatin in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Sensitivity: 24.7pg/ml Range: 61.7-5000pg/ml Specificity: This assay has high sensitivity and excellent specificity for detection of Obestatin (OB). No significant cross-reactivity or interference between Obestatin (OB) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to obestatin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled obestatin and unlabeled obestatin (Standards or samples) with the pre-coated antibody specific to obestatin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of obestatin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of obestatin in the sample. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1*120ul Detection Reagent B, 1*120ul TMB Substrate, 1*9ml Wash Buffer (30X), 1*20ml Standard Diluent, 1*20ml Assay Diluent A, 1*12ml Assay Diluent B, 1*12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
