Sphingosine-1-Phosphate (S1P) BioAssay(TM) ELISA Kit (General)

Product Overview

• Article Name: Sphingosine-1-Phosphate (S1P) BioAssay(TM) ELISA Kit (General)
• Biozol Catalog Number: USB-517659
• Supplier Catalog Number: 517659
• Alternative Catalog Number: USB-517659-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Intended Use: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of S1P in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Sensitivity: 4.62ng/ml Range: 12.35-1000ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of Sphingosine-1-Phosphate (S1P). No significant cross-reactivity or interference between Sphingosine-1-Phosphate (S1P) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to S1P has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled S1P and unlabeled S1P (Standards or samples) with the pre-coated antibody specific to S1P. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of S1P in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of S1P in the sample. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1*120ul Detection Reagent B, 1*120ul TMB Substrate, 1*9ml Wash Buffer (30X), 1*20ml Standard Diluent, 1*20ml Assay Diluent A, 1*12ml Assay Diluent B, 1*12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.