Interferon-Inducible Double Stranded RNA-Dependent Protein Kinase Activator A, Recombinant, Bovine, aa1-313, His-Tag, Myc-Tag (PRKRA)

Product Overview

• Article Name: Interferon-Inducible Double Stranded RNA-Dependent Protein Kinase Activator A, Recombinant, Bovine, aa1-313, His-Tag, Myc-Tag (PRKRA)
• Biozol Catalog Number: USB-517969
• Supplier Catalog Number: 517969
• Alternative Catalog Number: USB-517969-20,USB-517969-200
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at Lys-386 at Ser-392 respectively and enhances its activity in a EIF2AK2/PKR-dependent manner. Source: Recombinant full length protein corresponding to aa1-313 of bovine Interferon-Inducible Double Stranded RNA-Dependent Protein Kinase Activator A, fused to 10xHis-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Molecular Weight: ~39.4kD Amino Acid Sequence: MSQSRHRAAAPPMEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNMVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIEELSANGQYQCLAELSTSPITVCHGSGISCSSAQSDAAHNALQYLKIIAERK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 39.4
• UniProt: Q2HJ92
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.