Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family. Source: Recombinant protein corresponding to aa41-272 of zebrafish Interleukin 1, fused to 10xHis-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Molecular Weight: ~32.9kD Amino Acid Sequence: CDMHEGIRLEMWTSQHKMKQLVNVIIALNRMKHIKPQSTEFGEKEVLDMLMANVIQEREVNVVDSVPSYTKTKNVLQCTICDQYKKSLVRSGGSPHLQAVTLRAGSSDLKVRFSMSTYASPSAPATSAQPVCLGISKSNLYLACSPAEGSAPHLVLKEISGSLETIKAGDPNGYDQLLFFRKETGSSINTFESVKCPGWFISTAYEDSQMVEMDRKDTERIINFELQDKVRI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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