Metalloprotease LoiP, Recombinant, E. coli, aa19-252, His-Tag (loiP)
Biozol Catalog Number:
USB-518002
Supplier Catalog Number:
518002
Alternative Catalog Number:
USB-518002-20,USB-518002-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Metalloprotease that cleaves substrates preferentially between Phe-Phe residues. Plays a role in response to some stress conditions. Seems to regulate the expression of speB. Source: Recombinant protein corresponding to aa19-252 of E. coli Metalloprotease LoiP, fused to 6xHis-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~29kD Amino Acid Sequence: CQNMDSNGLLSSGAEAFQAYSLSDAQVKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAFAMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASAGGIVGSLSQSQLGNLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLAKLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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