U1 Small Nuclear Ribonucleoprotein 70kD, Recombinant, Human, aa1-210, His-Tag (SNRNP70)

Product Overview

• Article Name: U1 Small Nuclear Ribonucleoprotein 70kD, Recombinant, Human, aa1-210, His-Tag (SNRNP70)
• Biozol Catalog Number: USB-518145
• Supplier Catalog Number: 518145
• Alternative Catalog Number: USB-518145-20,USB-518145-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5 splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA. Source: Partial recombinant protein corresponding to aa1-210 of human U1 Small Nuclear Ribonucleoprotein 70kD, fused to 6xHis-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~28.7kD Amino Acid Sequence: MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRH Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 28.7
• UniProt: P08621
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.