Jacalin lectin (AIA) is purified by affinity chromatography and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the alpha, -chain. The proteins structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galbeta1,3GalNAc. Potent inhibitors of jacalin include D-galactose, beta-Met-Gal and 2-deoxy-alpha, -D-galactose. Source: Jacalin Blood Specificity: O (+SA), T antigen Sugar Specificity: Galbeta, 3GalNAc Inhibitory Carbohydrate: Galactose or Melibiose Divalent Ions: None Required Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
66
Purity:
Affinity purified
Form:
Supplied as a lyophilized powder.
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