Alkaline phosphatase (AP) is conjugated to Erythrina cristagalli (Coral tree) Lectin (ECL/ECA) to show the binding of ECL/ECA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140kD) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time. Source: Coral tree Blood Specificity: A (-SA) Sugar Specificity: Galbeta, 4GlcNAc Inhibitory Carbohydrate: Lactose Divalent Ions: Ca++, Mn++, Zn++ Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Purity:
Highly Purified
Form:
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Alkaline Phophatase (AP).
* VAT and and shipping costs not included. Errors and price changes excepted
