Alkaline phosphatase (AP) is conjugated to Griffonia simplicifolia Lectin (GSL II) to show the binding of GSL II in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140kD) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time. Source: Bandeiraea Blood Specificity: A (-SA) >> B (-SA) Sugar Specificity: alpha-N-Acetylglucosamine, beta-N-Acetylglucosamine Inhibitory Carbohydrate: Chitin Hydrolysate or N-Acetylglucosamine Divalent Ions: Ca++, Mn++ Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Form:
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Alkaline Phophatase (AP).
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