Cy5, when bound to Griffonia simplicifolia Lectin (GSL II), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens. Source: Bandeiraea Blood Specificity: A (-SA) >> B (-SA) Sugar Specificity: alpha-N-Acetylglucosamine, beta-N-Acetylglucosamine Inhibitory Carbohydrate: Chitin Hydrolysate or N-Acetylglucosamine Fluorescence: Red Divalent Ions: Ca++, Mn++ Excitation: 650nm Emission: 670nm Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months after receipt at 4C. CY5 conjugates are sensitive to light
Form:
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Cy5.
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