Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (alpha-1,3) and (alpha-1,6) linked mannosyl units of alpha-mannose residues. HHL reacts not only with terminal but also with internal alpha-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked alpha-mannosyl. HHL also binds to some yeast galactomannans. Carbohydrate Specificity: alpha-Mannose Inhibitory Carbohydrate: alpha(1,3) or alpha(1,6) linked mannosyl units Divalent Ions: None Required Activity: Agglutinates rabbit but not human erythrocytes Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
50
Purity:
Affinity purified
Form:
Supplied as a lyophilized powder.
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