Psophocarpus tetragonolobus lectin (PTL I, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by fractionation. The seed contains two lectins in which PTL I is the basic of the two. PTL I is a dimeric glycoprotein with specificity to GalNAc, with eluting sugar GalNAc. This lectin agglutinates trypsinized rabbit and human erythrocytes (type A and B) but not trypsinized human type O erythrocytes. PTL I recognize blood group A-substance derived oligosaccharides such as trisaccharide GalNAcalpha1-3Galbeta1-4Glc. Source: Winged bean Blood Specificity: Rabbit Agglutinin, O (- SA) Sugar Specificity: N-Acetylgalactosamine, Galactose Inhibitory Carbohydrate: N-Acetylgalactosamine Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
58
Purity:
Semi-Pure
Form:
Supplied as a lyophilized powder.
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