Horseradish peroxidase (HRP) is a 40kD protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Succinylated Triticum vulgaris Lectin (Succ WGA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics. Source: Wheat Blood Specificity: A, B, O Sugar Specificity: N-Acetylglucosamine Inhibitory Carbohydrate: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt Divalent Ions: Ca++ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Purity:
Highly Purified
Form:
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% thimerosal. Labeled with horseradish peroxidase (HRP).
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