Triticum vulgaris Lectin (WGA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers. Source: Wheat Blood Specificity: A, B, O Sugar Specificity: N-Acetylglucosamine Inhibitory Carbohydrate: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt Fluorescence: Green Divalent Ions: Ca++ Excitation: 495nm Emission: 515nm Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Affinity purified
Form:
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM calcium chloride, 0.5mM manganese chloride, 0.1% trehalose, 0.01% sodium azide, 5% glycerol . Labeled with Fluorescein isothiocyanate (FITC).
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