Cy3 can be used to visualize the binding pattern of Ulex europaeus Lectin (UEA I) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling. Source: Gorse Blood Specificity: O > A2 Sugar Specificity: alpha-Fucose Inhibitory Carbohydrate: L-Fucose Fluorescence: Orange Divalent Ions: Ca++, Mn++, Zn++ Excitation: 550nm Emission: 570nm Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. CY3 conjugates are sensitive to light.
Form:
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Cy3.
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