Cy5, when bound to Vicia villosa Lectin (VVL/VVA), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens. Source: Hairy vetch Sugar Specificity: N-Acetylgalactosamine Inhibitory Carbohydrate: N-Acetylgalactosamine Divalent Ions: Ca++, Mn++ Fluorescence: Dark purple Excitation (Maximum): 650nm Emission (Maximum): 670nm Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. CY5 conjugates are sensitive to light.
Form:
Supplied as a liquid in 10mM HEPES, 150mM sodium chloride, 0.05mM CaCl, 0.05mM MnCl, 1% BSA, 0.02% sodium azide. Labeled with Cy5.
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