ALT2/GPT2, Recombinant, Mouse, aa1-522, His-Tag

Product Overview

• Article Name: ALT2/GPT2, Recombinant, Mouse, aa1-522, His-Tag
• Biozol Catalog Number: USB-532255
• Supplier Catalog Number: 532255
• Alternative Catalog Number: USB-532255-50,USB-532255-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

ALT2/GPT2, also known as alanine aminotransferase, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. Source: Recombinant protein corresponding to aa1-522 from mouse ALT2/GPT2 fused to His-Tag at N-terminal expressed in E. coli. Molecular Weight: ~60.1kD (542aa) confirmed by MALDI-TOF Endotoxin: ~1EU/1ug (LAL) Biological Activity: Specific activity is > 50units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C. AA Sequence: MGSSHHHHHH SSGLVPRGSH MQRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS Applications: Suitable for use in Enzyme Activity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 60.1
• NCBI: 776291
• UniProt: Q8BGT5
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 2mM DTT, 20% glycerol.