Interleukin 1 alpha, Recombinant, Rat, aa115-270, His-Tag (Il1a, IL-1F1)

Product Overview

• Article Name: Interleukin 1 alpha, Recombinant, Rat, aa115-270, His-Tag (Il1a, IL-1F1)
• Biozol Catalog Number: USB-532331
• Supplier Catalog Number: 532331
• Alternative Catalog Number: USB-532331-100,USB-532331-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

B-cell maturation and proliferation, and fibroblast growth factor activity. IL1 proteins are involved in the inflammatory response, especially IL1A is an early and important mediator of inflammation after injury. Recombinant protein corresponding to aa115-270 from rat Interleukin 1 alpha, fused to His-Tag, expressed in E. coli. Swiss/Uniprot: P16598 Molecular Weight: ~20.2kD (179aa) confirmed by MALDI-TOF Biological Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 15ng/ml. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD RIHLKAASLN DLQLEVKFDM YAYSSGGDDSKYPVTLKVSN TQLFVSAQGE DKPVLLKEIP ETPKLITGSE TDLIFFWEKI NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS Applications: Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 20.2
• NCBI: 058715
• UniProt: P16598
• Purity: 95% (SDS-PAGE), Endotoxin: 1EU/ug (LAL).
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.