Neutrophil Elastase, Recombinant, Mouse, aa27-265, His-Tag
Catalog Number:
USB-532351
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| Article Name: | Neutrophil Elastase, Recombinant, Mouse, aa27-265, His-Tag |
| Biozol Catalog Number: | USB-532351 |
| Supplier Catalog Number: | 532351 |
| Alternative Catalog Number: | USB-532351-10,USB-532351-50 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Neutrophil elastase, also known as polymorphonuclear leukocyte eslastase, is a serine protease that classified as the chymotrypsin family. This protein breaks down elastin, an elastic fiber together with collagen and determines the mechanical properties of connective tissue. The neutrophil form breaks down the outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Also, this protein is inhibited by the acute-phase protein alpha 1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin. A1AT is normally secreted by the liver cells into the serum. Alpha-1-antitrypsin deficiency (A1AD) leads to uninhibited destruction of elastic fiber by elastase. Source: Recombinant protein corresponding to aa27-265 from mouse Neutrophil Elastase fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~26.8kDa (245aa) Endotoxin: ~1EU/1ug (LAL) AA Sequence: SEIVGGRPAR PHAWPFMASL QRRGGHFCGA TLIARNFVMS AAHCVNGLNF RSVQVVLGAH DLRRQERTRQ TFSVQRIFEN GFDPSQLLND IVIIQLNGSA TINANVQVAQ LPAQGQGVGD RTPCLAMGWG RLGTNRPSPS VLQELNVTVV TNMCRRRVNV CTLVPRRQAG ICFGDSGGPL VCNNLVQGID SFIRGGCGSG LYPDAFAPVA EFADWINSII RSHNDHLLTH PKDREGRTNH HHHHH Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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