SPARC, Recombinant, Human, aa18-303, His-Tag
Catalog Number:
USB-532379
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| Article Name: | SPARC, Recombinant, Human, aa18-303, His-Tag |
| Biozol Catalog Number: | USB-532379 |
| Supplier Catalog Number: | 532379 |
| Alternative Catalog Number: | USB-532379-50,USB-532379-100 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| SPARC, also known as osteonectin, is a member of secreted matricellular proteins. It is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. This protein regulates cell growth through interactions with the extracellular matrix and cytokines and binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. It functions as modulating cell-cell and cell-matrix interactions and its de-adhesive and growth inhibitory properties in non-transformed cell. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. A correlation between SPARC over-expression and ampullary cancers and chronic pancreatitis has been found. It also shows affinity for collagen in addition to bone mineral calcium. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, it is linked to human obesity. Recombinant protein corresponding to aa18-303 from human SPARC fused to His-tag at C-terminal, expressed in Baculovirus.. Molecular Weight: ~33.8kD (295aa) Amino Acid Sequence: ADPAPQQEAL PDETEVVEET VAEVTEVSVG ANPVQVEVGE FDDGAEETEE EVVAENPCQN HHCKHGKVCE LDENNTPMCV CQDPTSCPAP IGEFEKVCSN DNKTFDSSCH FFATKCTLEG TKKGHKLHLD YIGPCKYIPP CLDSELTEFP LRMRDWLKNV LVTLYERDED NNLLTEKQKL RVKKIHENEK RLEAGDHPVE LLARDFEKNY NMYIFPVHWQ FGQLDQHPID GYLSHTELAP LRAPLIPMEH CTTRFFETCD LDNDKYIALD EWAGCFGIKQ KDIDKDLVIH HHHHH Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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