Source: Recombinant protein corresponding to aa36-162 from woodchuck CTLA4, fused to Fc region of Human IgG1 and with a C-terminal Avi-Tag, expressed in a HEK293 cells. Molecular Weight: ~42kD Assay Conditions: Reaction done similar to human CTLA4:B7-1[biotin] BioAssay(TM) kit protocol. Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence. = Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
42
Purity:
90%
Form:
Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
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