Recombinant lectin protein with a Hexa-His Tag from the Rotavirus Sia3, expressed in E. coli. Purity: 95% (SDS PAGE analysis using Coomassie Blue detection and gel Permeation Chromatography) Form: Supplied as a liquid in PBS, pH 7.2. Specificity: Terminal alpha-linked Neu5Ac Molecular Weight: 19,584.89D (Monomer), Exists as a monomer under physiological conditions (PBS, pH 7.2) Usage Guidelines: RPL activity requires metal ions. All buffers must be supplemented with 1mM CaCl2, MgCl2 and MnCl2. Recommended dilution to desired working concenration (20ug/ml) in TBS, pH 7.6. Following dilution with TBS and supplemented with metal ions, the RPL should be incubated for a minimum of 30 minutes prior to use to allow the metal complexation. Theoretical Ext. Coefficient [E0.1%]: 1.755 Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
19584.89
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