CDC123 is a member of the CDC123 family. CDC123 is required for S phase entry of the cell cycle. It is also broadly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes with the uppermost expression in testis. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1011bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAAGAAGGAGCATGTGCTTCACTGCCAGTTCTCCGCGTGGTACCCGTTCTTCCGAGGCGTTACCATCAAGAGTGTCATTCTTCCACTTCCTCAGAATGTGAAGGATTATTTACTCGATGATGGAACTCTGGTGGTTTCAGGAAGGGATGATCCACCAACACATTCTCAGCCAGACAGTGATGATGAAGCAGAAGAAATACAGTGGTCTGATGATGAGAACACAGCCACGCTTACGGCACCAGAATTTCCTGAGTTTGCCACTAAAGTCCAGGAAGCTATCAATTCCCTCGGGGGCAGTGTCTTTCCTAAGCTTAATTGGAGTGCCCCAAGGGATGCGTATTGGATAGCAATGAATAGTTCTCTGAAATGTAAAACCCTCAGCGACATCTTTCTGCTTTTCAAGAGTTCCGATTTCATCACTCGTGACTTCACTCAGCCGTTTATTCATTGTACTGATGATTCTCCAGATCCATGTATAGAATATGAGCTCGTTCTCCGAAAATGGTGTGAATTGATTCCTGGGGCTGAGTTTCGATGTTTTGTCAAGGAAAACAAGCTTATTGGTATTTCTCAAAGAGACTACACACAATACTATGATCATATTTCTAAACAAAAGGAAGAAATTCGCAGATGCATACAAGACTTTTTCAAGAAACACATACAGTACAAATTCTTAGATGAAGACTTTGTGTTCGATATATACAGAGACAGTAGGGGGAAGGTGTGGCTCATTGACTTTAATCCATTTGGTGAAGTCACAGATTCACTGCTGTTCACCTGGGAAGAACTGATATCTGAGAACAACTTAAACGGCGATTTTAGTGAAGTTGACGCTCAAGAGCAGGATTCCCCAGCTTTCCGTTGCACAAACAGTGAAGTGACAGTCCAGCCCAGCCCCTATTTGAGTTACCGGCTACCCAAGGACTTTGTAGACCTCTCTACTGGGGAGGACGCTCACAAGCTAATAGACTTCCTTAAGCTGAAGAGAAATCAGCAGGAGGACGACTGA Translation Sequence: MKKEHVLHCQ FSAWYPFFRG VTIKSVILPL PQNVKDYLLD DGTLVVSGRD DPPTHSQPDS DDEAEEIQWS DDENTATLTA PEFPEFATKV QEAINSLGGS VFPKLNWSAP RDAYWIAMNS SLKCKTLSDI FLLFKSSDFI TRDFTQPFIH CTDDSPDPCI EYELVLRKWC ELIPGAEFRC FVKENKLIGI SQRDYTQYYD HISKQKEEIR RCIQDFFKKH IQYKFLDEDF VFDIYRDSRG KVWLIDFNPF GEVTDSLLFT WEELISENNL NGDFSEVDAQ EQDSPAFRCT NSEVTVQPSP YLSYRLPKDF VDLSTGEDAH KLIDFLKLKR NQQEDD Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
