CDC37L1 is a cytoplasmic phosphoprotein that exists in complex with HSP90 (HSPCA, MIM 140571) as well as several other proteins involved in HSP90-mediated protein folding. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1014bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGAACAACCGTGGCCGCCTCCGGGACCCTGGAGCCTCCCTCGGGCCGAGGGTGAGGCTGAGGAAGAGAGTGACTTCGACGTGTTCCCCAGTTCTCCCCGCTGCCCGCAGCTGCCAGGCGGCGGCGCCCAGATGTATAGCCATGGAATTGAATTGGCTTGCCAAAAGCAGAAAGAGTTTGTGAAGAGCTCTGTGGCGTGCAAATGGAATCTTGCTGAAGCTCAACAGAAACTTGGTAGCTTAGCACTGCATAATTCTGAGTCCTTGGATCAGGAGCATGCCAAAGCACAAACAGCAGTATCAGAACTGAGGCAACGGGAAGAAGAGTGGCGACAGAAAGAAGAAGCTCTAGTACAAAGAGAGAAGATGTGTCTGTGGAGCACGGATGCCATTAGCAAGGATGTTTTTAATAAGAGTTTTATTAATCAAGATAAAAGAAAAGACACAGAAGATGAAGATAAATCAGAATCATTTATGCAAAAATATGAGCAAAAAATCAGACATTTTGGTATGTTGAGTCGATGGGATGATAGCCAGAGATTTTTGTCTGACCATCCATACCTTGTATGTGAAGAAACTGCTAAATATCTTATTTTATGGTGTTTTCACCTGGAAGCTGAGAAGAAAGGGGCTTTAATGGAACAAATAGCACATCAAGCTGTTGTAATGCAGTTTATTATGGAAATGGCCAAAAACTGTAATGTGGATCCAAGAGGGTGTTTTCGTTTATTTTTCCAGAAAGCCAAAGCAGAGGAAGAAGGTTATTTTGAAGCATTCAAAAATGAACTTGAAGCTTTCAAGTCAAGAGTAAGACTTTATTCTCAATCACAAAGTTTTCAACCTATGACAGTTCAGAATCATGTTCCCCATTCTGGTGTTGGATCTATAGGTTTATTAGAATCCTTACCACAGAATCCAGATTATCTTCAGTATTCTATCAGTACAGCTCTCTGCAGCTTAAACTCGGTGGTACATAAAGAAGATGATGAACCCAAAATGATGGACACTGTATAA Translation Sequence: MEQPWPPPGP WSLPRAEGEA EEESDFDVFP SSPRCPQLPG GGAQMYSHGI ELACQKQKEF VKSSVACKWN LAEAQQKLGS LALHNSESLD QEHAKAQTAV SELRQREEEW RQKEEALVQR EKMCLWSTDA ISKDVFNKSF INQDKRKDTE DEDKSESFMQ KYEQKIRHFG MLSRWDDSQR FLSDHPYLVC EETAKYLILW CFHLEAEKKG ALMEQIAHQA VVMQFIMEMA KNCNVDPRGC FRLFFQKAKA EEEGYFEAFK NELEAFKSRV RLYSQSQSFQ PMTVQNHVPH SGVGSIGLLE SLPQNPDYLQ YSISTALCSL NSVVHKEDDE PKMMDTV Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
