CIAO1 (Cytosolic Iron-Sulfur Assembly Component 1) is a component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. It seems to specifically modulate the transactivation activity of WT1. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1020bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAAGGACTCGCTGGTGCTGCTGGGCCGTGTCCCGGCGCACCCGGACTCCCGCTGCTGGTTCCTGGCCTGGAACCCCGCGGGGACCCTGCTGGCCTCGTGCGGCGGCGACCGGAGAATCCGCATCTGGGGCACGGAGGGTGACAGCTGGATCTGCAAGTCTGTCCTTTCTGAAGGCCACCAGCGCACCGTGCGGAAGGTAGCCTGGTCCCCCTGCGGTAATTACCTGGCCTCTGCCAGCTTTGATGCTACCACTTGCATTTGGAAGAAGAACCAGGATGACTTTGAGTGTGTAACCACTCTCGAGGGCCATGAAAATGAGGTCAAGTCAGTGGCTTGGGCCCCATCTGGCAACCTCCTGGCCACTTGCAGCCGAGATAAGAGCGTTTGGGTCTGGGAAGTTGATGAAGAGGATGAGTATGAATGTGTCAGTGTTCTCAACTCCCACACACAGGATGTCAAGCATGTGGTTTGGCACCCAAGTCAGGAGCTCTTAGCTTCTGCCAGCTATGATGACACAGTGAAGCTGTACCGGGAGGAAGAGGATGACTGGGTATGCTGTGCCACCCTTGAGGGCCATGAATCCACTGTGTGGAGCTTGGCCTTTGACCCGAGTGGCCAGCGCCTGGCGTCTTGTAGTGATGACCGTACTGTGCGTATCTGGCGTCAGTATCTACCAGGCAATGAACAAGGGGTGGCATGCAGCGGCTCTGACCCCAGTTGGAAATGTATCTGTACTTTGTCCGGCTTCCACTCAAGGACCATTTATGACATTGCTTGGTGTCAGCTGACAGGGGCTCTGGCCACAGCTTGTGGGGATGACGCGATCCGCGTGTTTCAGGAGGATCCCAACTCGGATCCACAGCAGCCCACCTTCTCCCTGACAGCCCACTTGCATCAGGCCCATTCCCAGGATGTCAACTGTGTGGCCTGGAACCCCAAGGAGCCAGGGCTACTGGCCTCCTGCAGTGATGATGGGGAGGTGGCCTTCTGGAAGTATCAGCGGCCTGAAGGCCTCTGA Translation Sequence: MKDSLVLLGR VPAHPDSRCW FLAWNPAGTL LASCGGDRRI RIWGTEGDSW ICKSVLSEGH QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT LEGHENEVKS VAWAPSGNLL ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT QDVKHVVWHP SQELLASASY DDTVKLYREE EDDWVCCATL EGHESTVWSL AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS WKCICTLSGF HSRTIYDIAW CQLTGALATA CGDDAIRVFQ EDPNSDPQQP TFSLTAHLHQ AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WKYQRPEGL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
