CNN1, also known as calponin-1, belongs to the calponin family. CNN1 is thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 894bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTCCTCTG CTCACTTCAA CCGAGGCCCT GCCTACGGGC TGTCAGCCGA GGTTAAGAAC AAGCTGGCCC AGAAGTATGA CCACCAGCGG GAGCAGGAGC TGAGAGAGTG GATCGAGGGG GTGACAGGCC GTCGCATCGG CAACAACTTC ATGGACGGCC TCAAAGATGG CATCATTCTT TGCGAATTCA TCAATAAGCT GCAGCCAGGC TCCGTGAAGA AGATCAATGA GTCAACCCAA AATTGGCACC AGCTGGAGAA CATCGGCAAC TTCATCAAGG CCATCACCAA GTATGGGGTG AAGCCCCACG ACATTTTTGA GGCCAACGAC CTGTTTGAGA ACACCAACCA TACACAGGTG CAGTCCACCC TCCTGGCTTT GGCCAGCATG GCGAAGACGA AAGGAAACAA GGTGAACGTG GGAGTGAAGT ACGCAGAGAA GCAGGAGCGG AAATTCGAGC CGGGGAAGCT AAGAGAAGGG CGGAACATCA TTGGGCTGCA GATGGGCACC AACAAGTTTG CCAGCCAGCA GGGCATGACG GCCTATGGCA CCCGGCGCCA CCTCTACGAC CCCAAGCTGG GCACAGACCA GCCTCTGGAC CAGGCGACCA TCAGCCTGCA GATGGGCACC AACAAAGGAG CCAGCCAGGC TGGCATGACT GCGCCAGGGA CCAAGCGGCA GATCTTCGAG CCGGGGCTGG GCATGGAGCA CTGCGACACG CTCAATGTCA GCCTGCAGAT GGGCAGCAAC AAGGGCGCCT CGCAGCGGGG CATGACGGTG TATGGGCTGC CACGCCAGGT CTACGACCCC AAGTACTGTC TGACTCCCGA GTACCCAGAG CTGGGTGAGC CCGCCCACAA CCACCACGCA CACAACTACT ACAATTCCGC CTAG Translation Sequence: MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
