CPPED1 (Calcineurin-Like Phosphoesterase Domain Containing 1) belongs to the metallophosphoesterase superfamily. This protein binds 2 divalent metal cations and is transactivated by the large envelope protein of the hepatitis B virus (HBV). Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 945bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTCGGCTGCAGAGGCGGGGGGTGTTTTCCACAGAGCCAGGGGCAGGACCCTGGCCGCGTTTCCCGCAGAAAAGGAAAGCGAATGGAAAGGCCCATTCTACTTCATCCTGGGCGCAGACCCACAGTTTGGGCTGATCAAGGCCTGGTCCACTGGGGACTGTGACAATGGCGGTGACGAATGGGAACAGGAGATCCGTCTAACTGAGCAAGCCGTCCAGGCCATCAACAAGCTGAACCCCAAACCCAAATTCTTCGTTCTGTGCGGCGACCTCATCCACGCCATGCCAGGGAAGCCGTGGCGGACGGAGCAGACGGAGGACCTGAAGCGAGTGCTTAGGGCAGTGGACAGGGCCATCCCACTGGTCCTTGTCAGCGGCAACCATGACATTGGCAACACCCCCACGGCCGAGACCGTCGAGGAGTTCTGCCGGACTTGGGGAGATGACTACTTCAGCTTCTGGGTCGGGGGCGTCCTGTTCCTGGTCCTCAACTCCCAGTTCTACGAGAACCCCTCCAAATGCCCCAGCCTGAAGCAGGCTCAGGACCAGTGGCTGGACGAGCAGCTGAGCATCGCGAGGCAGCGGCACTGCCAGCATGCCATCGTCTTCCAGCACATCCCGCTGTTCCTGGAGAGCATCGACGAGGACGACGACTACTACTTCAACCTCAGCAAGTCCACTCGGAAGAAGTTGGCAGACAAGTTCATCCACGCAGGTGTCAAAGTCGTGTTCTCAGGCCACTACCACAGGAATGCCGGGGGTACCTACCAGAACCTCGACATGGTGGTGTCATCTGCCATTGGATGCCAGCTGGGCAGAGACCCCCACGGGCTCCGAGTCGTGGTGGTCACCGCCGAGAAAATTGTTCACCGATACTACAGTCTAGATGAGCTGAGTGAGAAAGGAATAGAAGACGATCTCATGGATTTGATCAAGAAAAAATGA Translation Sequence: MSAAEAGGVF HRARGRTLAA FPAEKESEWK GPFYFILGAD PQFGLIKAWS TGDCDNGGDE WEQEIRLTEQ AVQAINKLNP KPKFFVLCGD LIHAMPGKPW RTEQTEDLKR VLRAVDRAIP LVLVSGNHDI GNTPTAETVE EFCRTWGDDY FSFWVGGVLF LVLNSQFYEN PSKCPSLKQA QDQWLDEQLS IARQRHCQHA IVFQHIPLFL ESIDEDDDYY FNLSKSTRKK LADKFIHAGV KVVFSGHYHR NAGGTYQNLD MVVSSAIGCQ LGRDPHGLRV VVVTAEKIVH RYYSLDELSE KGIEDDLMDL IKKK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
