CPXCR1 is one of several genes identified in a region of the X chromosome associated with an X-linked cleft palate (CPX) disorder. Mutation analysis of this gene has not revealed any mutation which causes the CPX disorder. Multiple alternatively spliced variants, encoding the same protein, have been identified. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 906bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAGTTCTCCTACTAAAGAAGGAAGTGATACAGCTGGAAATGCTCACAAAAATTCTGAAAATGAGCCTCCTAATGACTGTAGTACAGACATAGAGTCTCCATCTGCTGATCCCAATATGATCTATCAGGTAGAAACCAACCCAATAAACAGGGAGCCAGGCACAGCAACCTCCCAGGAAGATGTTGTTCCTCAAGCAGCAGAAAACAGCGAGCTCGAAACAGAGATCCAAAAAGATCAACGAGAAGAAGATCTAAAAGAAGAGCTTCTTCTACTTCAGACCCCCATTCCCAGAAAATTGGTCTCTCACAAGCCCTTAAATGATAGATCAAGATCCCACTCAGGGAAAGTTGAGATGAAAGCAAACAATTTCCCCATAAATCACAAAACTCGTTTTCGACTTTCAACTTCATGGAGAGTCCCATTTATTAACAGTCATGAGATAAGAAGTATGATTCTCCATCTGCTATGTGATAGATATTTCTCTCAGGCTGCAGGGTGTCAGAATACCATGTGGGTAAAGCGAAAATATATAGCATGTCTTTACCATCCAAATAGTTTCACCCATCACGAGAGAGCCATAACATTTAGAAGGCCTTCGAGGGTGCACTACTACCGTCCCCTCACTGAGAGAATGACATCAGGAAAATTTTGCAAATCAACTGACACTAAAGGGAAATGTAGATTCCGTGCTATTGTGAGGTCTGTGCTCTTTGTGTCACAGATACAAATTGAAAGTATTTTTAATATAAAAGGTTTTGTGGATATATTGACATATATCCATACCATGAATGTTATGATCACAAACACCAATAATGGTTGGAAATACTTTTGTCCCATCTGTGGAAGGCTTTTTAACACTTACTCTGAATTAAGACAACATTCATGCAGCTCTTCTGGGAATTAA Translation Sequence: MSSPTKEGSD TAGNAHKNSE NEPPNDCSTD IESPSADPNM IYQVETNPIN REPGTATSQEDVVPQAAENS ELETEIQKDQ REEDLKEELL LLQTPIPRKL VSHKPLNDRS RSHSGKVEMKANNFPINHKT RFRLSTSWRV PFINSHEIRS MILHLLCDRY FSQAAGCQNT MWVKRKYIACLYHPNSFTHH ERAITFRRPS RVHYYRPLTE RMTSGKFCKS TDTKGKCRFR AIVRSVLFVSQIQIESIFNI KGFVDILTYI HTMNVMITNT NNGWKYFCPI CGRLFNTYSE LRQHSCSSSGN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
