CUEDC1 contains 1 CuE domain. The CuE domain is structurally related to the ubiquitin-binding uBA domain and has been reported to exist as a domain-swapped dimer that makes additional contacts with ubiquitin, and consequently, binds ubiquitin with higher affinity. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1161bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGACCAGCCTGTTCCGCCGGAGCAGCAGCGGCAGCGGCGGGGGTGGCACCGCCGGGGCACGCGGGGGCGGGGGAGGCACGGCCGCCCCCCAGGAGCTCAACAACAGCCGGCCTGCCCGCCAGGTGCGCCGCCTGGAGTTCAACCAGGCCATGGACGACTTCAAGACCATGTTCCCCAACATGGATTACGACATCATCGAATGCGTGCTGCGCGCCAACAGCGGCGCTGTGGACGCCACCATCGACCAGCTGCTGCAGATGAACCTGGAGGGCGGTGGCAGCAGCGGCGGCGTCTATGAGGACAGCTCCGACTCGGAGGACAGCATCCCCCCGGAGATCTTGGAAAGGACTTTGGAACCTGATAGCTCGGATGAAGAGCCCCCACCTGTGTACTCCCCGCCAGCCTACCACATGCACGTGTTCGACCGGCCCTACCCTCTGGCTCCCCCGACTCCGCCTCCCCGTATCGACGCGCTGGGCTCTGGAGCCCCTACAAGCCAGAGACGCTATCGGAACTGGAACCCACCACTGCTGGGCAACCTTCCGGATGACTTTCTCCGCATCCTGCCCCAGCAGCTGGACAGCATACAGGGTAACGCTGGGGGCCCCAAGCCTGGGAGTGGAGAGGGATGTCCACCTGCCATGGCTGGGCCAGGGCCCGGAGACCAGGAGAGCCGCTGGAAGCAGTACCTGGAGGACGAGAGGATCGCGCTTTTCCTGCAGAACGAGGAGTTCATGAAGGAGCTGCAACGGAACCGCGACTTCCTCCTCGCTCTGGAGAGAGATCGATTGAAATACGAATCCCAGAAATCTAAATCCAGCAGCGTGGCTGTCGGAAACGACTTTGGCTTTTCCTCTCCTGTCCCAGGAACTGGCGACGCCAACCCCGCTGTGTCTGAAGATGCCTTATTCAGGGACAAGCTGAAACACATGGGAAAGTCCACCCGGAGGAAACTGTTTGAACTTGCCCGAGCCTTCTCAGAGAAGACCAAAATGAGGAAGTCAAAGAGGAAACACTTGTTGAAGCATCAGTCGCTGGGGGCTGCCGCGTCAACAGCCAACCTCCTGGATGATGTGGAGGGCCACGCGTGTGATGAAGACTTCCGGGGCAGGCGTCAGGAGGCACCCAAGGTGGAGGAAGGCCTGCGAGAAGGACAGTAA Translation Sequence: MTSLFRRSSS GSGGGGTAGA RGGGGGTAAP QELNNSRPAR QVRRLEFNQA MDDFKTMFPN MDYDIIECVL RANSGAVDAT IDQLLQMNLE GGGSSGGVYE DSSDSEDSIP PEILERTLEP DSSDEEPPPV YSPPAYHMHV FDRPYPLAPP TPPPRIDALG SGAPTSQRRY RNWNPPLLGN LPDDFLRILP QQLDSIQGNA GGPKPGSGEG CPPAMAGPGP GDQESRWKQY LEDERIALFL QNEEFMKELQ RNRDFLLALE RDRLKYESQK SKSSSVAVGN DFGFSSPVPG TGDANPAVSE DALFRDKLKH MGKSTRRKLF ELARAFSEKT KMRKSKRKHL LKHQSLGAAA STANLLDDVE GHACDEDFRG RRQEAPKVEE GLREGQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
