DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178aa allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to duMP, thereby producing the nucleotide substrate (duMP) that is used by thymidylate synthase. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 537bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAGTGAAG TTTCCTGCAA GAAACGGGAC GACTATTTGG AATGGCCAGA GTATTTTATG GCTGTGGCCT TCTTATCAGC ACAGAGAAGC AAAGATCCAA ATTCCCAGGT CGGCGCCTGC ATCGTGAATT CAGAAAACAA GATTGTCGGG ATTGGGTACA ATGGGATGCC AAATGGGTGC AGTGATGACG TGTTGCCTTG GAGAAGGACA GCAGAGAATA AGCTGGACAC CAAATACCCG TACGTGTGCC ATGCGGAGCT GAATGCCATC ATGAACAAAA ATTCGACCGA TGTGAAAGGC TGTAGTATGT ATGTTGCCTT GTTCCCTTGT AATGAATGCG CTAAGCTCAT CATCCAGGCA GGTATAAAAG AAGTGATTTT CATGTCTGAT AAATACCATG ATAGTGACGA GGCAACTGCT GCGAGGCTCC TGTTTAATAT GGCCGGGGTG ACATTCCGGA AATTCATACC GAAGTGCAGC AAGATTGTCA TTGACTTTGA TTCAATTAAC AGCAGACCGA GTCAAAAGCT TCAGTGA Translation Sequence: MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
