DHRS12 is a member of the short-chain dehydrogenases/reductases (SDR) family, which has over 46,000 members. Members in this family are enzymes that metabolize many different compounds, such as steroid hormones, prostaglandins, retinoids, lipids and xenobiotics. Alternative splicing results in multiple transcript variants and protein isoforms. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 816bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAATCTGCATGTAAAGACTTTGTCCCTCATGACTTGGAGGTCCAGATTCCTGGAAGAGTCTTTTTGGTCACTGGAGGAAACAGCGGCATTGGCAAAGCAACTGCCCTTGAAATCGCCAAGCGAGAACATTTTTCTGCACATTGTGGACTTGTCTGATCCCAAGCAAATCTGGAAATTTGTTGAAAATTTCAAGCAGGAACATAAACTCCATGTTCTGATCAATAATGCAGGTTGCATGGTCAATAAAAGAGAGCTCACAGAAGATGGACTTGAAAAAAACTTTGCTGCCAATACTCTGGGTGTGTACATTCTCACGACCGGCCTGATCCCTGTGCTGGAGAAAGAACACGACCCCCGAGTGATAACCGTCTCCTCAGGAGGAATGTTGGTTCAGAAACTGAACACCAATGATCTCCAGTCCGAAAGAACACCATTTGATGGAACTATGGTCTATGCACAAAACAAGAGGCAGCAAGTGGTTCTGACGGAGCGGTGGGCCCAAGGGCACCCGGCCATCCATTTTTCTTCCATGCATCCTGGCTGGGCCGACACCCCAGACAGGAATGAGCAGGAGCTGAGGAAGGTAGTGGGAGAGGCCCAGACTGCCTCACCACTCCCCAGGTTTTTGGAAATAATGATGCATGAAGGTAAATGCCAGCCACAAGGACACAGCTCGAATGATCTGGAAGCGTGTTGGAGCAGCGGTGGAGGGGAGCAGAATTCTCTTCCGGATTGGCCTCACCAACTCCATGACCTCAGGCAGCTCACCTGGGCTCTCTGCAGCTCTTTCCTCCTCTACAAACAAGGGAACTGA Translation Sequence: MNLHVKTLSL MTWRSRFLEE SFWSLEETAA LAKQLPLKSP SENIFLHIVD LSDPKQIWKFVENFKQEHKL HVLINNAGCM VNKRELTEDG LEKNFAANTL GVYILTTGLI PVLEKEHDPRVITVSSGGML VQKLNTNDLQ SERTPFDGTM VYAQNKRQQV VLTERWAQGH PAIHFSSMHPGWADTPDRNE QELRKVVGEA QTASPLPRFL EIMMHEGKCQ PQGHSSNDLE ACWSSGGGEQNSLPDWPHQL HDLRQLTWAL CSSFLLYKQG N Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
