Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 783bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCCTCCT TGGAAGTCAG TCGTAGTCCT CGCAGGTCTC GGCGGGAGCT GGAAGTGCGC AGTCCACGAC AGAACAAATA TTCGGTGCTT TTACCTACCT ACAACGAGCG CGAGAACCTG CCGCTCATCG TGTGGCTGCT GGTGAAAAGC TTCTCCGAGA GTGGAATCAA CTATGAAATT ATAATCATAG ATGATGGAAG CCCAGATGGA ACAAGGGATG TTGCTGAACA GTTGGAGAAG ATCTATGGGT CAGACAGAAT TCTTCTAAGA CCACGAGAGA AAAAGTTGGG ACTAGGAACT GCATATATTC ATGGAATGAA ACATGCCACA GGAAACTACA TCATTATTAT GGATGCTGAT CTCTCACACC ATCCAAAATT TATTCCTGAA TTTATTAGGA AGCAAAAGGA GGGTAATTTT GATATTGTCT CTGGAACTCG CTACAAAGGA AATGGAGGTG TATATGGCTG GGATTTGAAA AGAAAAATAA TCAGCCGTGG GGCCAATTTT TTAACTCAGA TCTTGCTGAG ACCAGGAGCA TCTGATTTAA CAGGAAGTTT CAGATTATAC CGAAAAGAAG TTCTAGAGAA ATTAATAGAA AAATGTGTTT CTAAAGGCTA CGTCTTCCAG ATGGAGATGA TTGTTCGGGC AAGACAGTTG AATTATACTA TTGGCGAGGT TCCAATATCA TTTGTGGATC GTGTTTATGG TGAATCCAAG TTGGGAGGAA ATGAAATAGT ATCTTTCTTG AAAGGATTAT TGACTCTTTT TGCTACTACA TAA Translation Sequence: MASLEVSRSP RRSRRELEVR SPRQNKYSVL LPTYNERENL PLIVWLLVKS FSESGINYEI IIIDDGSPDG TRDVAEQLEK IYGSDRILLR PREKKLGLGT AYIHGMKHAT GNYIIIMDAD LSHHPKFIPE FIRKQKEGNF DIVSGTRYKG NGGVYGWDLK RKIISRGANF LTQILLRPGA SDLTGSFRLY RKEVLEKLIE KCVSKGYVFQ MEMIVRARQL NYTIGEVPIS FVDRVYGESK LGGNEIVSFL KGLLTLFATT Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
