ETEA, also designated UBX domain-containing protein 8 (UBXD8), is a 445aa protein associated with atopic dermatitis (AD), a chronic, noncontagious, relapsing inflammatory skin disease characterized by eczematous skin lesions also referred to as eczematous dermatitis. ETEA may be involved in the resistance to apoptosis in T cells and eosinophils of AD patients. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 1338bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCGGCGCCTGAGGAGCGGGATCTAACCCAGGAGCAGACAGAGAAGCTGCTGCAGTTTCAGGATCTCACTGGCATCGAATCTATGGATCAGTGTCGCCATACCTTGGAACAGCATAACTGGAACATAGAGGCTGCTGTACAGGACAGATTGAATGAGCAAGAGGGCGTACCTAGTGTTTTCAACCCACCTCCATCACGACCCCTGCAGGTTAATACAGCTGACCACAGGATCTACAGCTATGTTGTCTCAAGACCTCAACCAAGGGGGCTGCTTGGATGGGGTTATTACTTGATAATGCTTCCATTCCGGTTTACCTATTACACGATACTTGATATATTTAGGTTTGCTCTTCGTTTTATACGGCCTGACCCTCGCAGCCGGGTCACTGACCCCGTTGGGGACATTGTTTCATTTATGCACTCTTTTGAAGAGAAATATGGGAGGGCACACCCTGTCTTCTACCAGGGAACGTACAGCCAGGCACTTAACGATGCCAAAAGGGAGCTTCGCTTTCTTTTGGTTTATCTTCATGGAGATGATCACCAGGACTCTGATGAGTTTTGTCGCAACACACTCTGTGCACCTGAAGTTATTTCACTAATAAACACTAGGATGCTCTTCTGGGCATGCTCTACAAACAAACCTGAGGGATACAGGGTCTCACAGGCTTTACGAGAGAACACCTATCCATTCCTGGCCATGATTATGCTGAAGGATCGAAGGATGACTGTGGTGGGACGGCTAGAAGGCCTCATTCAACCTGATGACCTCATTAACCAACTGACATTTATCATGGATGCTAACCAGACTTACCTGGTGTCAGAACGCCTAGAAAGGGAAGAAAGAAACCAGACCCAAGTGCTGAGACAACAGCAGGATGAGGCCTACCTGGCCTCTCTCAGAGCTGACCAGGAGAAAGAAAGAAAGAAACGGGAGGAGCGGGAGCGTAAGCGGCGGAAGGAGGAGGAGGTGCAACAGCAAAAGTTGGCAGAGGAGAGACGGCGGCAGAATTTACAGGAGGAAAAGGAAAGGAAGTTGGAATGCCTGCCCCCTGAACCTTCCCCTGATGACCCTGAAAGTGTCAAGATCATCTTCAAATTACCTAATGATTCTCGAGTAGAGAGACGATTCCACTTTTCACAGTCTCTAACAGTAATCCACGACTTCTTATTCTCCTTGAAGGAAAGCCCAGAAAAGTTTCAGATTGAAGCCAATTTTCCCAGGCGAGTGCTGCCCTGCATCCCTTCAGAGGAGTGGCCCAATCCCCCTACGCTACAGGAGGCCGGACTCAGCCACACAGAAGTTCTTTTTGTTCAGGACCTAACTGACGAATGA Translation Sequence: MAAPEERDLT QEQTEKLLQF QDLTGIESMD QCRHTLEQHN WNIEAAVQDR LNEQEGVPSV FNPPPSRPLQ VNTADHRIYS YVVSRPQPRG LLGWGYYLIM LPFRFTYYTI LDIFRFALRF IRPDPRSRVT DPVGDIVSFM HSFEEKYGRA HPVFYQGTYS QALNDAKREL RFLLVYLHGD DHQDSDEFCR NTLCAPEVIS LINTRMLFWA CSTNKPEGYR VSQALRENTY PFLAMIMLKD RRMTVVGRLE GLIQPDDLIN QLTFIMDANQ TYLVSERLER EERNQTQVLR QQQDEAYLAS LRADQEKERK KREERERKRR KEEEVQQQKL AEERRRQNLQ EEKERKLECL PPEPSPDDPE SVKIIFKLPN DSRVERRFHF SQSLTVIHDF LFSLKESPEK FQIEANFPRR VLPCIPSEEW PNPPTLQEAG LSHTEVLFVQ DLTDE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
