GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Vector Description: GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. cDNA Size: 1140bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGTCGCGCA GTGGAACCGA TCCTCAGCAA CGCCAGCAGG CGTCAGAGGC GGACGCCGCA GCAGCAACCT TCCGGGCAAA CGACCATCAG CATATCCGCT ACAACCCGCT GCAGGATGAG TGGGTGCTGG TGTCAGCTCA CCGCATGAAG CGGCCCTGGC AGGGTCAAGT GGAGCCCCAG CTTCTGAAGA CAGTGCCCCG CCATGACCCT CTCAACCCTC TGTGTCCTGG GGCCATCCGA GCCAACGGAG AGGTGAATCC CCAGTACGAT AGCACCTTCC TGTTTGACAA CGACTTCCCA GCTCTGCAGC CTGATGCCCC CAGTCCAGGA CCCAGTGATC ATCCCCTTTT CCAAGCAAAG TCTGCTCGAG GAGTCTGTAA GGTCATGTGC TTCCACCCCT GGTCGGATGT AACGCTGCCA CTCATGTCGG TCCCTGAGAT CCGGGCTGTT GTTGATGCAT GGGCCTCAGT CACAGAGGAG CTGGGTGCCC AGTACCCTTG GGTGCAGATC TTTGAAAACA AAGGTGCCAT GATGGGCTGT TCTAACCCCC ACCCCCACTG CCAGGTATGG GCCAGCAGTT TCCTGCCAGA TATTGCCCAG CGTGAGGAGC GATCTCAGCA GGCCTATAAG AGTCAGCATG GAGAGCCCCT GCTAATGGAG TACAGCCGCC AGGAGCTACT CAGGAAGGAA CGTCTGGTCC TAACCAGTGA GCACTGGTTA GTACTGGTCC CCTTCTGGGC AACATGGCCC TACCAGACAC TGCTGCTGCC CCGTCGGCAT GTGCGGCGGC TACCTGAGCT GACCCCTGCT GAGCGTGATG ATCTAGCCTC CATCATGAAG AAGCTCTTGA CCAAGTATGA CAACCTCTTT GAGACGTCCT TTCCCTACTC CATGGGCTGG CATGGGGCTC CCACAGGATC AGAGGCTGGG GCCAACTGGA ACCATTGGCA GCTGCACGCT CATTACTACC CTCCGCTCCT GCGCTCTGCC ACTGTCCGGA AATTCATGGT TGGCTACGAA ATGCTTGCTC AGGCTCAGAG GGACCTCACC CCTGAGCAGG CTGCAGAGAG ACTAAGGGCA CTTCCTGAGG TTCATTACCA CCTGGGGCAG AAGGACAGGG AGACAGCAAC CATCGCCTGA Translation Sequence: MSRSGTDPQQ RQQASEADAA AATFRANDHQ HIRYNPLQDE WVLVSAHRMK RPWQGQVEPQ LLKTVPRHDP LNPLCPGAIR ANGEVNPQYD STFLFDNDFP ALQPDAPSPG PSDHPLFQAK SARGVCKVMC FHPWSDVTLP LMSVPEIRAV VDAWASVTEE LGAQYPWVQI FENKGAMMGC SNPHPHCQVW ASSFLPDIAQ REERSQQAYK SQHGEPLLME YSRQELLRKE RLVLTSEHWL VLVPFWATWP YQTLLLPRRH VRRLPELTPA ERDDLASIMK KLLTKYDNLF ETSFPYSMGW HGAPTGSEAG ANWNHWQLHA HYYPPLLRSA TVRKFMVGYE MLAQAQRDLT PEQAAERLRA LPEVHYHLGQ KDRETATIA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
