The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4, MIM 610611), Psf1 (GINS1, MIM 610608), Psf2, and Psf3 (GINS3, MIM 610610). The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 558bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGACGCTGCCGAGGTCGAATTCCTCGCCGAGAAGGAGCTGGTTACCATTATCCCCAACTTCAGTCTGGACAAGATCTACCTCATCGGGGGGGACCTGGGGCCTTTTAACCCTGGTTTACCCGTGGAAGTGCCCCTGTGGCTGGCGATTAACCTGAAACAAAGACAGAAATGTCGCCTGCTCCCTCCAGAGTGGATGGATGTAGAAAAGTTGGAGAAGATGAGGGATCATGAACGAAAGGAAGAAACTTTTACCCCAATGCCCAGCCCTTACTACATGGAACTTACGAAGCTCCTGTTAAATCATGCTTCAGACAACATCCCGAAGGCAGACGAAATCCGGACCCTGGTCAAGGATATGTGGGACACTCGTATAGCCAAACTCCGAGTGTCTGCTGACAGCTTTGTGAGACAGCAGGAGGCACATGCCAAGCTGGATAACTTGACCTTGATGGAGATCAACACCAGCGGGACTTTCCTCACACAAGCGCTCAACCACATGTACAAACTCCGCACGAACCTCCAGCCTCTGGAGAGTACTCAGTCTCAGGACTTCTAG Translation Sequence: MDAAEVEFLA EKELVTIIPN FSLDKIYLIG GDLGPFNPGL PVEVPLWLAI NLKQRQKCRL LPPEWMDVEK LEKMRDHERK EETFTPMPSP YYMELTKLLL NHASDNIPKA DEIRTLVKDM WDTRIAKLRV SADSFVRQQE AHAKLDNLTL MEINTSGTFL TQALNHMYKL RTNLQPLEST QSQDF Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
