GLIPR2 is closely related to plant pathogenesis-related (PR-1) proteins, which are upregulated in response to pathogen attack. This protein is found within lipid-enriched microdomains on the cytosolic side of the endomembrane system. GLIPR2 is tightly anchored to membranes and absent from the cytosol, although it does not possess a membrane-spanning domain. Vector Description: This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 465bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGGCAAGT CAGCTTCCAA ACAGTTTCAT AATGAGGTCC TGAAGGCCCA CAATGAGTAC CGGCAGAAGC ACGGCGTCCC CCCACTGAAG CTCTGCAAGA ACCTCAACCG GGAGGCTCAA CAGTATTCTG AGGCCCTGGC CAGCACGAGG ATCCTCAAGC ACAGCCCGGA GTCCAGCCGT GGCCAGTGTG GGGAGAACCT TGCATGGGCA TCCTATGATC AGACAGGAAA GGAGGTGGCT GATAGATGGT ACAGTGAAAT CAAGAACTAT AACTTCCAGC AGCCTGGCTT CACCTCGGGG ACTGGACACT TCACGGCCAT GGTATGGAAG AACACCAAGA AGATGGGCGT GGGGAAGGCG TCCGCAAGTG ACGGGTCCTC CTTTGTGGTG GCCAGATACT TCCCAGCGGG GAATGTTGTC AATGAGGGCT TCTTCGAAGA AAACGTCCTG CCGCCGAAGA AGTAA Translation Sequence: MGKSASKQFH NEVLKAHNEY RQKHGVPPLK LCKNLNREAQ QYSEALASTR ILKHSPESSR GQCGENLAWA SYDQTGKEVA DRWYSEIKNY NFQQPGFTSG TGHFTAMVWK NTKKMGVGKA SASDGSSFVV ARYFPAGNVV NEGFFEENVL PPKK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
