GULP1 is an adapter protein necessary for the engulfment of apoptotic cells by phagocytes. Several transcript variants, some protein coding and some thought not to be protein coding, have been found for this gene. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 915bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGAACCGTGCTTTTAGCAGGAAGAAAGACAAAACATGGATGCATACACCTGAAGCTTTATCAAAACATTTCATTCCCTATAATGCAAAGTTTCTTGGCAGTACAGAAGTGGAACAGCCAAAAGGAACAGAAGTTGTGAGAGATGCTGTAAGGAAACTAAAGTTTGCAAGACATATCAAGAAATCTGAAGGCCAGAAAATTCCTAAAGTGGAGTTGCAAATATCAATTTATGGAGTAAAAATTCTAGAACCCAAAACAAAGGAAGTTCAACACAATTGCCAGCTTCATAGAATATCTTTTTGTGCAGATGATAAAACTGACAAGAGGATATTCACTTTCATATGCAAAGATTCTGAGTCAAATAAACATTTGTGCTATGTATTTGACAGCGAAAAGTGTGCTGAAGAGATCACTTTAACAATTGGCCAAGCATTTGACCTGGCATACAGGAAATTTCTAGAATCAGGAGGAAAAGATGTTGAAACAAGAAAACAGATCGCAGGGTTACAAAAAAGAATCCAAGACTTAGAAACAGAAAATATGGAACTTAAAAATAAAGTACAAGATTTGGAAAACCAACTGAGAATAACTCAAGTATCAGCACCTCCAGCAGGCAGTATGACACCTAAGTCGCCCTCCACTGACATCTTTGATATGATTCCATTTTCTCCAATATCACACCAGTCTTCGATGCCTACTCGCAATGGCACACAGCCACCTCCAGTACCTAGTAGATCTACTGAGATTAAACGGGACCTGTTTGGAGCAGAACCTTTTGACCCATTTAACTGTGGAGCAGCAGATTTCCCTCCAGATATTCAATCAAAATTAGATGAGATGCAGGAGGGGTTCAAAATGGGACTAACTCTTGAAGGCACAGTATTTTGTCTCGACCCGTTAGACAGTAGGTGCTGA Translation Sequence: MNRAFSRKKD KTWMHTPEAL SKHFIPYNAK FLGSTEVEQP KGTEVVRDAV RKLKFARHIK KSEGQKIPKV ELQISIYGVK ILEPKTKEVQ HNCQLHRISF CADDKTDKRI FTFICKDSES NKHLCYVFDS EKCAEEITLT IGQAFDLAYR KFLESGGKDV ETRKQIAGLQ KRIQDLETEN MELKNKVQDL ENQLRITQVS APPAGSMTPK SPSTDIFDMI PFSPISHQSS MPTRNGTQPP PVPSRSTEIK RDLFGAEPFD PFNCGAADFP PDIQSKLDEM QEGFKMGLTL EGTVFCLDPL DSRC Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
