HCK encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector. cDNA Size: 459bp Preparation before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid. Nucleotide Sequence: ATGGCCAACC TGGAGCGCAC CTTCATCGCC ATCAAGCCGG ACGGCGTGCA GCGCGGCCTG GTGGGCGAGA TCATCAAGCG CTTCGAGCAG AAGGGATTCC GCCTCGTGGC CATGAAGTTC CTCCGGGCCT CTGAAGAACA CCTGAAGCAG CACTACATTG ACCTGAAAGA CCGACCATTC TTCCCTGGGC TGGTGAAGTA CATGAACTCA GGGCCGGTTG TGGCCATGGT CTGGGAGGGG CTGAACGTGG TGAAGACAGG CCGAGTGATG CTTGGGGAGA CCAATCCAGC AGATTCAAAG CCAGGCACCA TTCGTGGGGA CTTCTGCATT CAGGTTGGCA GGAACATCAT TCATGGCAGT GATTCAGTAA AAAGTGCTGA AAAAGAAATC AGCCTATGGT TTAAGCCTGA AGAACTGGTT GACTACAAGT CTTGTGCTCA TGACTGGGTC TATGAATAA Translation Sequence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
